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Tamayo develops a serum with the obtained blood samples to cure Nezuko, who is kept isolated far from them as she recovers. Muzan appears and Kagaya triggers a suicide attack to stagger him. The Hashira ambush Muzan but he traps them all within the Infinity Castle, an endless labyrinth which houses Akaza, Upper Two Doma, Upper One Kokushibo, and the newly-ascended members Kaigaku and Nakime; Zenitsu's former partner and Muzan's personal assistant, respectively. As Zenitsu kills Kaigaku, Tamayo injects Muzan with a poison she and Shinobu have made to weaken his abilities. When Akaza remembers his human life, he betrays Muzan and commits suicide, while Shinobu sacrifices herself to poison Doma, allowing Inosuke and fellow Demon Slayer Kanao Tsuyuri to kill him. After a harrowing battle, Kokushibo kills Muichiro and Genya before being killed as well. Muzan kills Tamayo, and later Nakime when she is hypnotized by Yushiro to use her powers against him, but is forced above ground by the Corps. Aided by Tamayo's poison, the Corps unleash a desperate battle of attrition as the remaining members fight against Muzan until the morning sun can kill him. Muzan is left helpless against the sun but Gyomei, Obanai, and Mitsuri succumb to their injuries. As Tanjiro delivers the final blow, a dying Muzan fatally wounds him and forcefully gives him his remaining blood. Tanjiro transforms into the ultimate being in Muzan's last-ditch effort to have his species survive.