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Sakonji has hypnotized Nezuko to not harm humans and she aids Tanjiro in dire situations as he starts his work of hunting down and slaying demons. One of their assignments bring them to Asakusa where they encounter Muzan Kibutsuji, the progenitor of all demons and the one responsible for the murder of their family. Tanjiro and Nezuko also meet Tamayo and Yushiro, two demons free from Muzan's control. They ally to develop a cure for Nezuko, though it will require Tanjiro to supply Tamayo with blood samples from the Twelve Kizuki, the twelve most powerful demons under Muzan's command, split into the six Lower Ranks and the six Upper Ranks. Along their journey, Tanjiro and Nezuko meet fellow Demon Slayers Zenitsu Agatsuma and Inosuke Hashibira; they team up after Tanjiro's mission to kill Kyogai, the former Lower Six. In his next mission, they join other Demon Slayers in facing the family of Lower Five Rui. In the midst of battle, Tanjiro unlocks a mysterious Breathing Style known as "Hinokami Kagura", but is ultimately outmatched by Rui, while Inosuke and Zenitsu are injured and the expedition is slaughtered. Giyu and Insect Hashira Shinobu Kocho rescue the survivors, who are brought back to the Corps' headquarters. Tanjiro is trial by the Hashira – Giyu, Shinobu, Flame Hashira Kyojuro Rengoku, Sound Hashira Tengen Uzui, Love Hashira Mitsuri Kanroji, Stone Hashira Gyomei Himejima, Mist Hashira Muichiro Tokito, Serpent Hashira Obanai Iguro, and Wind Hashira Sanemi Shinazugawa – who, with the exception of Giyu and Mitsuri, do not believe he and Nezuko should be allowed to live. Nonetheless, the Corps' leader Kagaya Ubuyashiki, is able to convince them to accept Nezuko and see Tanjiro's importance, believing it is with their help that Muzan can be defeated. Upon learning of Rui's death, Muzan kills the remaining Lower Ranks due to their constant failures; he spares Lower One Enmu and sends him on a mission to kill Tanjiro.
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